Cell Culture and Cell-Based Assays

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Who Should Attend: This practical course is for everyone who deals with cultured mammalian cells. Absolute beginners but also experienced cell culture enthusiasts will benefit from this training. Scientists and technicians working in assay development or routine potency testing should attend, as well as lab managers overseeing the cell depository and cell banking activities.

Click on the titles below to learn more about each session. When live sessions are available, sign up anytime and receive links to recordings of any individual sessions or all five sessions. Although designed to be taken in a series, you may pick and choose individual sessions, as they are designed to be stand-alone units. When you are ready, click on the “Register Now” button to sign up for individual sessions or the full course.

Each 60-minute session is just $339 per session or $1696 for all five sessions! Great group discounts are available.

Session 1: Introduction into Mammalian Cell Culture

Session 1: Introduction into Mammalian Cell Culture

  • Instructor: Dr. Oliver Wehmeier
  • Format: On-demand recording available now.
  • Content:
    ● Historical background of cell culture
    ● Morphologies and tissues
    ● Primary cells vs. immortal cells
    ● Cell aging & cell bank management

Session 2: Maintenance of Cultured Cells

Maintenance of Cultured Cells

  • Instructor: Dr. Oliver Wehmeier
  • Format: On-demand recording available now.
  • Content:
    ● Techniques of cell cultivation, culture vessels
    ● Adherent vs. suspension growth
    ● Media and supplements, serum-free options
    ● Cell passaging: harvesting, counting, seeding
    ● Cryopreservation

Session 3: Contaminations in Cell Culture

Contamination in Cell Culture

  • Instructor: Dr. Oliver Wehmeier
  • Format: On-demand recording available now.
  • Content:
    ● Types & sources of contamination
    ● Cross contamination & cell identity
    ● Detection and “treatment”
    ● Aseptic working, antibiotics

Session 4: Genetic Modifications

Genetic Modifications

  • Instructor: Dr. Oliver Wehmeier
  • Format: On-demand recording available now.
  • Content:
    ● Transient vs. stable transfection
    ● Vector systems & transfection methods
    ● Viral transduction
    ● Selected pools vs. clonal cell lines
    ● Constitutive vs. inducible expression

Session 5: Cell-Based Assays

Cell-Based Assays

  • Instructor: Dr. Oliver Wehmeier
  • Format: On-demand recording available soon.
  • Content:
    ● Mechanism of action and end points
    ● Assay technologies
    ● Assay parameters: Seeding density, incubation, read-out
    ● Assay Ready Cells

COPYRIGHT: U.S. Copyright Law protects the program you are about to attend from unauthorized duplication. Multiple participants are not authorized to share access provided to a single registrant.  For each individual who attends, a single dedicated seat license must be purchased, or a group rate must have been previously arranged with FasTrain. FasTrain reserves the right, at its discretion, to cancel or interrupt access to a web-based training class without notice, or to invoice and collect the group rate payment for the class from the single registrant if this requirement has been violated.